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Emerging platforms such as three-dimensional cultures, patient-derived organoids, and patient-derived xenografts (PDX) offer improved preservation of tumor architecture, metabolic gradients, and subtype-specific features

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For LNA (18:3) dose response assays, overnight cultures were diluted to 1x10 6 cells/mL and 250 μL of the cell dilution was pipetted onto SC+uri agar plates