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49 The diversity of responses to insulin observed between cell lines in our study, eg, in H2AX phosphorylation or DNA repair gene expression, may be partially explained by differences in the frequency of NGFR-positive cells

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Approximately 5 × 10 4 cells/well were distributed in pre-coated 96-well plates and allowed to adhere overnight (37 °C, 5% CO 2 )