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The restriction sites were introduced by PCR using the primer pairs 5′-TGGATCCGCTCAAGAGTTTGTGAACTG-3′ and 5′-CTAAGCTTTTACTTGTACAGCTCGTCC-3′ for GRX1-roGFP2iL and 5′-AAGGATCCGTGAGCAAGGGCGAGGAGC-3′ and 5′-CTAAGCTTTTACTTGTACAGCTCGTCC-3′ for roGFP2-iL
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