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由于反应为可逆反应，若温度过高，会使得产品分解，降低产率；若温度过低，会大大延长反应时间。Since the reaction is a reversible reaction, if the temperature is too high, the product will be decomposed and the yield will be reduced

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To quantify the SCFA concentrations in the colonic luminal contents, the samples were homogenised with 150 mm-NaHCO 3 (pH 7·8) (1:5, w/v) in an Ar atmosphere