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When B12 comes into our body, it's attached to proteins in the food we eat
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Experimental αA (1-11: MDVTIQHPWFK), βB2 (4-14: HQTQAGKPQSL), and γS (52-71: PNFAGYMYILPQGEYPEYQR) human crystallin peptides in their native and two potentially deamidated forms (synthesized by AnaSpec, Fremont, CA, USA) were analyzed by ECD at ∼10 -5 M concentration in the spray solution containing 50:50 (v/v) methanol: water with 1% formic acid