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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20ยฐC, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4ยฐC, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)
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A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration
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