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7, the exposure to LFUS resulted in enhancing the uptake from both the non-conjugated DPPA and DPPA-TRA, with the highest increase in drug uptake recorded in the cells incubated with DPPA-TRA and sonicated with LFUS showing a 33.4% increase in calcein fluorescence value (140,474 ± 329 A.U.) compared to those incubated with DPPA-TRA with no sonication (100,230 ± 2783 A.U., p = 3.3 × 10 –5 ) and a 136.6% increase in calcein uptake compared to those incubated with control DPPA without sonication (26,451 ± 902 A.U., p = 3.04 × 10 –5 )