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Effect of oxygen on induction of the cystine transporter by bacterial lipopolysaccharide in mouse peritoneal macrophages
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To initiate the transfection process, a mixture containing HO-1 siRNA or HA-tagged HMOX1 plasmid, Opti-MEM (Gibco, Grand Island, NY, USA), and Lipofectamine 3000 (Invitrogen, Groningen, Netherlands) was incubated at room temperature for 15 min
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