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After washing several times in 0.01 M PBS, the samples were treated with anti-rabbit IgG antibodies (Thermo Fisher Scientific) in 0.01 M PBS at 22 ± 2 °C for 1 h

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In this post, we’ll break down the four stages of sleep , explain why each is important, and—most importantly—share how to optimize each stage using science-backed strategies and functional medicine tools