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After phase separation, the top aqueous layer containing NAD + was recovered and the pH was corrected by addition of 1 M Tris (pH 8.0)

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(Swaminathan et al., 2017), the X-ray crystal structure of a ternary complex of NNMT bound with MNAM was elucidated, revealing that MNAM binds to the active site of NNMT, thereby impeding NAM binding and suppressing NNMT’s enzymatic activity