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After extensive washing with PBS and then NH 4 CO 3, bound proteins were eluted with 100 mM DTT, concentrated 5-fold and applied to 12% SDS-PAGE gels
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The vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), endothelin 1 (ET-1), e-selectin, inducible nitric oxide synthase (iNOS), TNF-α, IL-6, and IL-1β levels were lowered after SYR treatment, while the levels of NO and nuc-p65 were enhanced
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