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In flies expressing mutant let-7 ( UAS-mut-let-7 ), which was generated by site-directed mutagenesis of the UAS-let-7 construct, with G-C transitions at seed sequence sites 3-4 of the mature let-7 miRNA and complementary mutations on the opposing side of the hairpin 32 (Supplementary Fig
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In addition, a specific miRNA may exert multiple regulatory effects by binding to several targets of metabolic signaling pathways, illustrated by the example of let-7 that binds to 3’UTR regions of INSR, IRS-2, and IGF-1R and regulates their expression (95)
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