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Ingredients that differed by five or more dog foods when compared across 3P/FDA and non-3P/FDA diet groups were considered “distinguishing ingredients.” Statistical analyses Linear regression was used to assess associations between the 3P/FDA and non-3P/FDA diet groups and the individual biochemical compounds present in the foods

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To the tissue lysate, ATP monitoring enzyme was added and the luminescent reaction was immediately analyzed in a microplate reader (Spectra Max MII, Molecular Devices, Germany)

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