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The PCR products were purified via extraction from a 2% agarose gel (QIAEX II gel extraction kit, QIAGEN #20021) and then assembled with the GPS lentiviral vector cut BstBI (NEB, #R0519S) and XhoI (NEB, #R0146S) along with the corresponding wild-type fragment(s) of CDO1 required to reconstitute full-length CDO1

canbediscounted

Size:40ML

there were only significant increases in Ct.Th and Ct.Ar