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To prepare the Transwell assay, murine melanoma cells (1 × 10 5 cells per insert) cultured in 200 μl RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 μm pores, Corning), while 800 μl RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber
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What glutathione does in the body Glutathione is a powerful antioxidant that the body makes naturally
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