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Identification of genes involved in the decrease of opacity using microarrays To identify the genes associated with the development of lens opacity induced by galactose and the effect of glutamate on opacity reduction, we cultured 2 samples (Control) for 4 and 6 days without galactose, 3 samples (Galactose) were cultured for 6 days in a medium containing galactose to induce opacity, and 2 samples (Glutamate) were cultured in a medium containing galactose for 3 days to induce opacity, followed by the addition of glutamate and an additional 3 days of culture for microarray analysis
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