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In a study on MTX‐induced hepatotoxicity in mice, oral administration of punicalagin (25 or 50 mg/kg/day) for 10 days prior to a single intraperitoneal MTX challenge (20 mg/kg) significantly reduced serum transaminases, ALP, and LDH levels, as well as hepatic oxidative stress markers, while enhancing antioxidant defenses in the liver [117]