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For immunohistochemical analysis, sagittal sections were stained against cell-specific marker proteins: rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, DakoCytomation, Glostrup, Denmark), mouse anti-glutamine synthetase (GS) (1:500, BD, Franklin Lakes, NJ, USA) and rabbit anti-S100B (1:500, Abcam, Cambridge, UK)
b12 and niacin injection
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