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Following this, the tissue samples were washed with PBS and incubated with avidin-biotin-peroxidase solution (ABC Elite Kit, Vector Labs, Burlingame, United States, 1:500) diluted in 0.4% Triton X-100 with NaCl and PBS for an additional 2 h
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Controlled release of IGF-I from a biodegradable matrix improves functional recovery of skeletal muscle from ischemia/reperfusion
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