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Although both GFP-ASXL1 1-645 and GFP-ASXL1 1-591 constructs contain ASXL1 EBM , occupancy of BRD4 on the PGK1 and VIM promoters in GFP-ASXL1 1-591 -WT expressing HEK293T cells was decreased compared to the occupancy of BRD4 on these promoters in GFP-ASXL1 1-645 -WT expressing cells (Fig