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Soft-agar clonogenicity assay Firstly, we mixed 3.5% low melting point agarose (#16520050, Thermo Fisher Scientific) and media (the volume ratio of agarose and media is 1: 5) to prepare the bottom soft-agar and then added it into 12-well plates quickly (1 mL soft-agar/well)
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Beyond these functions, ATM regulates peroxisome and mitochondrial function 8,9 and impacts glucose import (GLUT1 pS490) and the glycolysis/pentose phosphate pathway (PPP) switch (HSP27:G6PD), particularly in response to exogenous damage 10,11