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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20°C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4°C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)
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