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Given the importance of this organelle, maintenance of proper mitochondrial function and structure is essential, and dysfunction is a pathological hallmark of metabolic, muscular, and neurodegenerative conditions, which can be associated with aging in multiple tissues 88,89

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73 , 80 , 113 , 161 To prevent the misassignment of generic ROS nanotoxicity as ferroptosis, we recommend including iron-salt comparators (equimolar Fe), reporting trafficking/lysosomal dwell time versus cytosolic access, and using autophagy inhibitors/rescues alongside ferroptosis-specific controls where appropriate