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We verified both ectopic integration of F-SNIP1 variants by Sanger sequencing of their genomic DNA and F-SNIP1 expression by western blotting with SNIP1 antibody (after induction with 1 μg/ml doxycycline for 24 h)

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2.9 Flow cytometric cell sorting analysis Following the procedure described in Section 2.2, HUVEC cells were digested and collected using trypsin without EDTA