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This occurs since AFB1 can impair the growth of follicles, the survival of oocytes, and the proliferation of granulocytes while reducing the levels of gonadotropin (30)

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The next day, slides were washed with TBST, followed by staining with the secondary antibodies of goat anti-rat IgG conjugated with TRITC (Jackson Lab) and goat anti-mouse IgG conjugated with FITC