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The SUMO protein (11 kDa) is involved in post-translational modification in eukaryotic cells, enhances solubility and stability of the binding partner when fused to the N-terminal (Marblestone et al., 2006), and allows simultaneous cleavage as it is recognized by the SUMO protease ( Saccharomyces cerevisiae Ulp1) at the conserved dipeptide glycine–glycine (Li and Hochstrasser, 1999)