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The staining was performed by incubation 20 min at 37 °C in red phenol free DMEM containing 20 mM Hepes, 50 nM TMRM and and 200 nM Mitotracker green.The samples were then mounted in PBS containing Calcium Magnesium and 10 mM glucose in a thermoregulated chamber, humidified and containing 5% CO2, of a Zeiss confocal microscope
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AT III restored colon p-AMPK and SIRT1 levels in DSS-treated animals [70]
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